Treat mice with chemical

Identify and count clones by hybridization
with probes from most common cDNAs.
Remove clones using robotics.

Begin high throughput sequencing
of unidentified clones.

Determine EST frequency by counting clones
identified by sequencing and hybridization.

Make cDNA microarrays using unique clones.

Search for gene expression patterns.
Test models of toxicity.

Apply custom microarrays to
additional chemical exposures.