Edge^2 environment

Environment, Drugs and Gene Expression

RNA Submission

Submission of total RNA for Bradfield Lab Microarray

  1. RNA must be greater than .600ug/ul in concentration and the total amount of RNA should be greater than 60ug/sample for each comparison. Control RNA that is to be used for multiple comparisons, please provide enough control RNA for each comparison (e.g. three dosed individuals against one control; provide 180ug of control)
  2. Each tube should be labeled with lab name, sample name, and RNA concentration.
  3. All packages should include a description sheet of each sample along with proof of RNA quality, either in a gel image or 260/280 ratio. Please include a primary contact name on the description sheet, along with phone number and address.
  4. Fill out an online submission form for EACH (distinct) RNA sample-->Online Form
  • IMPORTANT: Microarray comparisons are made between untreated, control animals and animals treated with ONE treatment. Please make sure the RNA submitted adheres to this experimental design. If you have any questions, please contact us.